Bl2b-t101c
Web1 h. The precipitate was pelleted by centrifugation at 15,000 g for 30 min.ThesupernatantwasdecantedandthepelletwasresuspendedinTris buffer using a 37°C water bath ... WebThe PCR reaction procedure involved initial denaturation at 95 °C for 5 min followed by 35 cycles of 95 °C for 30 sec (denaturing), annealing at 55 °C (bl2b_tem1, bl2be_shv2, and vang for 1 min; arna for 2 min; and rosb for 1.5 min), and 72 °C for 30 sec (extension), with a final extension step for 7 min at 72 °C.
Bl2b-t101c
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