Callus induction benthamiana
WebDec 1, 2024 · Callus used for genetic transformation was derived from mini-kumquat, they were cultured on MT (Murashge-Tucker) medium (4.4 g/L MT Base Salts with vitamins, 4% [w/v] Sucrose and 0.8% [w/v] agar, pH5.8) under normal light conditions (8 h/16 h night/day) at 25 °C. Citrus callus transformation Webcallus [kal´us] 1. localized hyperplasia of the horny layer of the epidermis due to pressure or friction. 2. an unorganized network of woven bone formed about the ends of a broken …
Callus induction benthamiana
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WebDec 7, 2024 · To examine the effect of PSK on adventitious shoot formation in protoplast-derived calluses of N. benthamiana, 70–150 cell aggregates from each plate were transferred to a plastic Petri dish (FALCON, 60 × 15 mm) containing 2 mL of solid shoot induction medium supplemented with each of the final concentrations (0, 0.01, 0.03, … WebDec 16, 2024 · Callus-like ‘bumps’ begin to appear, typically on cotyledons in N. benthamiana and on hypocotyls in tomatoes, roughly 12 d after the removal of A. tumefaciens (Supplementary Fig. 1b–d). Over ...
WebJul 21, 2024 · Vitis vinifera plants are disease-susceptible while Vitis pseudoreticulata plants are disease-resistant; however, the molecular mechanism remains unclear. In this study, the single-stranded DNA- and RNA-binding protein gene Whirly (VvWhy1 and VpWhy1) were cloned from V. vinifera “Cabernet Sauvignon” and V. pseudoreticulata “HD1”. WebCallus induction and shoots regeneration from callus was obtained by using MS medium modified with 1-naphthalene acetic acid (auxin) and 6-benzylaminopurine (cytokinin). …
WebMES Monohydrate (solid) 30.00 g. Agar - Plant Cell Culture (solid) 2000.0 ul. (2,4-Dichlorophenoxy)acetic Acid Sodium Salt, 1 mg/ml. Add after autoclaving other ingredients and cooling to ~60°C. 2000.0 ul. Kinetin, 0.1 mg/ml. Add after autoclaving other ingredients and cooling to ~60°C. Webcallus: [noun] a thickening of or a hard thickened area on skin or bark.
WebMay 15, 2008 · This paper reports the growth characteristics, measurement of fresh weight and dry weight of callus of tobacco (Nicotiana tabacum) cells grown on Murashige and …
WebJan 21, 2024 · First, the callus induction was performed on MS medium supplemented with 1 mg/L 2,4-D, 0.1 mg/L NAA, 0.2 mg/ L 6-BA, 0.01 mg/L TDZ, 0.1 g/L Tim, 0.1 g/L Cefo, … take center console apart 2014 vw passatWebSep 29, 2024 · Here, we describe a method for highly efficient targeted mutagenesis in Nicotiana benthamiana through the expression of Cas9 and single-guide (sg)RNA using a potato virus X ... surface-sterilized leaves at 7 dai were placed onto callus and shoot induction medium (Fig. 2A). Cefotaxime was added to the medium to kill Agrobacterium, ... take cell phone on planeWe previously demonstrated that the four Arabidopsis LBD transcription factors (LBD16, LBD17, LBD18 and LBD29) act downstream of ARF7 and ARF19 to direct auxin-induced callus formation15. To gain further insight into the molecular control of auxin-induced callus formation, we used LBD17 as a bait and … See more To examine whether AtbZIP59, as a partner of LBDs, is also involved in directing auxin-induced callus formation, we obtained a transfer DNA (T-DNA) insertion mutant of … See more To investigate the tissue-specific expression of AtbZIP59, we generated transgenic Arabidopsis plants harbouring a ProAtbZIP59::GUS transgene. A GUS staining assay … See more Since AtbZIP59 and LBD could form a heterodimer, it is likely that they share a common downstream target to direct callus formation. A … See more To further explore the interactive role of AtbZIP59 and LBD in auxin-induced callus formation, we generated a lbd16-2 air1-2 double mutant and examined its callus-forming capacity. … See more take cell phone screenshot