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Glut1 western blot protocol

WebApr 12, 2024 · To determine if OBHS’s ability to induce the inactivation of the Akt/mTOR pathway in MCF-7 cells is correlated with its inhibition of GLUT1, we performed Western blot analysis, which showed the downregulated expression of p-Akt after OBHS treatment, and unfortunately, there was no significance in mTOR phosphorylation (Figure 2G). WebShowing 21 Protocols. Number. Description. 7442. Western Blotting Protocol: Mini-PROTEAN TGX Stain-Free Gel/Trans-Blot Turbo/EveryBlot Blocking/Chemiluminescence. 7443. Western Blotting Protocol: Mini-PROTEAN TGX Stain-Free Gel/Trans-Blot Turbo/EveryBlot Blocking/Fluorescence. 7441.

GLUT1 - Wikipedia

WebDec 14, 2024 · f Western blot of GLUT1 and TRIM38 proteins in WCLs from T24 cells transfected with indicated plasmids with DMSO, MG132 (20 μM) or with Bortezmib (20 nM) for 12 h. g RT-qPCR assessment of GLUT1 mRNA expression in T24 cells treated with DMSO, MG132 (20 μM) or with Bortezmib (20 nM) for 12 h. The mRNA level of GAPDH … WebDec 9, 2024 · (A) Western blot analysis of glucose transporter and glycolytic enzyme levels in PyVT tumor cells ectopically expressing c-Myc. (B) Immunofluorescent staining of the Glut1 transporter in PyVT tumor cells ectopically expressing c-Myc. Scale bar, 100 μm. tower of fantasy esturion de fabry https://1stdivine.com

Need help with detecting Glucose Transporter 1 in …

WebLane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is Glut1 (E4S6I) Rabbit mAb. Western blot analysis was performed using Glut1 … WebWestern Blot protocol for Glut1 Antibody (NB110-39113) [email protected] Protocols, Publications, Related Products, Reviews and more: www.novusbio.com/NB110-39113 Orders: NB110-39113 Support: [email protected] Web: … WebJun 29, 2024 · The scale bar indicates 50 μm. (C) Western blot analysis of GLUT1 expression in control, Ret∆Glut1, and Rod∆Glut1 retinas (5 μg/well). Blots are representative of N = 3 mice. Quantification of relative expression of GLUT1 in retinas of control, Ret∆Glut1, and Rod∆Glut1. The intensity of GLUT1 was normalized to Vinculin. power automate build url

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Glut1 western blot protocol

Recombinant Anti-Glucose Transporter GLUT1 antibody [SP168 …

Web(A and B) Western blot (A) and image analysis of the blot (B) provides evidence of reduced Glut1 in brain capillary fractions of 5-month-old Tie2-Cre;Glut1 fl/+ mutants. (C) Western blot showing equivalent levels of Glut1 in brain neuropil fractions of Tie2-Cre;Glut1 fl/+ mutants and controls. WebAug 21, 2024 · f Representative western blot showing the variable RB1 expression levels ... ascorbic acid, l-glutamine, and FBS as per the manufacturer’s protocol. After 24 h, BAY-876 was added to the plate at ...

Glut1 western blot protocol

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http://www.diva-portal.org/smash/get/diva2:748080/FULLTEXT01.pdf WebDiscard the supernatant. 3. Wash the cells once by resuspending the cell pellet in ice-cold PBS. Pellet cells by centrifugation at 2,500 x g for 10 minutes. 4. Add ice-cold …

WebDescription. RNase H，即核糖核酸酶H，是一种核糖核酸内切酶，可特异性地水解DNA-RNA结合链中RNA磷酸二酯键。. RNase H具有双二价金属离子催化机制，如Mg2+和Mn2+直接参与催化功能。. RNase H不能水解单链或双链DNA或RNA中的磷酸二酯键。. 本试剂盒可用于cDNA第二链合成前 ... WebJun 16, 2016 · I have no idea. Here is the protocol I have been following: Block in 5% skim milk after transfer to PVDF membrane. Incubate with primary antibody overnight (12-20h) …

WebProduct Specific Protocols; WB protocol for GLUT1 antibody 66290-1-Ig: Download protocol: IHC protocol for GLUT1 antibody 66290-1-Ig: Download protocol: ... NIH/3T3 cells were subjected to SDS PAGE … WebWestern Blotting Protocol. For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween ® 20 at 4°C with gentle shaking, overnight. NOTE: Please refer to primary antibody product webpage for recommended antibody dilution. A. Solutions and Reagents

WebJun 22, 1998 · In the present study, we examined the cellular localization of GLUT1 in the rat brain by double immunostaining methods and immunoelectron microscopic analysis using a rabbit antiserum specific to GLUT1. Western blot analysis of the rat brain revealed that the antiserum detected a strong band with a molecular weight of 45 kDa and a weak …

WebArticle Title: Downregulating hypoxia‐inducible factor‐2α improves the efficacy of doxorubicin in the treatment of hepatocellular carcinoma Journal: Cancer Science doi: 10.1111/j.1349-7006.2011.02177.x tower of fantasy euríaleWebPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Notes; ICC/IF 1/100. Flow Cyt 1/200. WB 1/200. Predicted molecular weight: 54 kDa. IHC-P ... Western blot - Anti-Glucose Transporter GLUT1 antibody [SP168] (ab150299) Anti-Glucose Transporter GLUT1 antibody [SP168] (ab150299) … power automate build jsonWebI am using following conditions for culture. 1- Proliferation medium is 10% FBS, 1% PS in DMEM High glucose and cells were cultured in 100mm plates. At 50% confluency, cells … power automate button in excel