WebbThe test method is based on reconstructed human epidermis (RhE), which in its overall design mimics the biochemical and physiological properties of the upper layers of the human skin. The test item is applied directly to the skin surface, providing a good model of “real life” exposure. Webb19 juni 2024 · Reconstruction of human cultured epidermis is achieved by cultivating proliferating keratinocytes on an inert filter substrate with a surface area of 0.3 cm 2 at the air-liquid interface for 13 days using an optimized medium containing 5% …

OECD Guidelines for the Testing of Chemicals, Section 4

Webb1 feb. 2010 · The SkinEthic™ Reconstructed Human Epidermal (RHE) skin irritation method is an internationally accepted OECD test method used to detect the skin irritation properties of chemicals (OECD TG 439) and has been shown to be robust and transferable. WebbONE catch-up validation students on modified human epidermis (SkinEthic HM RHE) for entire replacement of the Draize skin irritation test. N. Alépée, HUNDRED ... Use of … 高校 サッカー 選手権 千葉 決勝 チケット

The epiCS® skin irritation test (SIT) method Request PDF

WebbMethod Description This method is based on the in vitro test system of reconstructed human epidermis (RhE), which closely mimics the biochemical and physiological properties of the upper parts of the human skin, i.e. the epidermis. WebbMethod Description The test method is based on the commercially available EpiDerm™ model, a reconstructed human epidermal (RhE) model grown from human-derived epidermal keratinocytes, which are cultured to form a multilayered and highly differentiated model of the human epidermis. WebbThe test material (solid or liquid) is applied uniformly and topically to a three-dimensional human skin model, comprising at least a reconstructed epidermis with a functional … tarteria adeje