Web15 Sep 2014 · Bovine serum albumin (BSA) 1 is an acidic single polypeptide protein with molecular weight of 66.4 kDa that has been used extensively in the pharmaceutical industry for a variety of applications. It is used as a stabilizer, a blocking agent in various immunoassays, in microarray technologies, and in cell culture medium as a growth … BSA is often used a model for other serum albumin proteins, especially human serum albumin, to which it is 76% structurally homologous. BSA has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry. Because BSA is a small, stable, moderately non-reactive protein, it is often used as a blocker in immunohistochemistry. During immunohist…
Role of echocardiography on early diagnosis of atrial remodelling …
WebELISA procedures Indirect ELISA was performed to determine the antibody titers (Le et al., 2009). TES-OVA were coated into a 96-well microplate (100 mL/well) for one hour at 378C. After three washes with PBS Tween 20 (PBST) (0.5% Tween 20), the wells were blocked with 1% BSA in PBS overnight at 48C and washed again with PBST. Then 50 Web28 Jan 2024 · Updated on January 28, 2024. PBS or phosphate-buffered saline is a buffer solution that is particularly valuable because it mimic the ion concentration, osmolarity, and pH of human body fluids. In other words, it's isotonic to human solutions, so it's less likely to cause cell damage, toxicity, or unwanted precipitation in biological, medical ... primark faux leather trousers
Comparison of Blocking Agents for ELISA - Thermo Fisher Scientific
Web1 day ago · Proteases are a family of proteolytic enzymes that play fundamental roles in the ... Bovine Serum Albumin (BSA) was obtained from Tocris (Bristol, UK). ... (DY511), Human Cathepsin-B DuoSet ELISA ... WebBovine Serum Albumin (BSA), molecular biology grade Pub. No. MAN0011930 Rev. Date 22 September 2024 (Rev. C.00) #B14 Assembling Lot 00000000 Filling Lot 00000000 Expiry Date MM.YYYY Store at -20 °C Components #B14 Bovine Serum Albumin (BSA), molecular biology grade, 20 mg/mL 5 mg www.thermofisher.com For Research Use Only. Web26 Mar 2015 · Pros of BSA Good general blocking agent Clearer results as it contains only one protein so less for the antibody to cross-react with. Works better with phospho-antibodies as albumin is a secreted protein and it tend to not be phosphorylated Cons of BSA More expensive than milk Should also be filtered to remove particulates primark faux leather coat