WebDec 1, 2016 · Botulinolysin (BLY) is a toxin produced by Clostridium botulinum that belongs to a group of thiol-activated hemolysins. In this study, a protein exhibiting hemolytic activity was purified from the culture supernatant of C. botulinum serotype D strain 4947. The purified protein displayed a single band by sodium dodecyl sulfate polyacrylamide gel … WebJan 26, 2013 · The toxin has a thiol-activated cytolysin which causes lysis of human platelets, lysosomes and a variety of subcellular membranes. The existing therapy seems to have challenged as available vaccines are not so effective and the bacteria developed resistance to many drugs. Computer aided approach is a novel platform to screen drug …
(PDF) Structural and functional features of Streptolysin O
The thiol-activated Cholesterol-dependent Cytolysin (CDC) family (TC# 1.C.12) is a member of the MACPF superfamily. Cholesterol dependent cytolysins are a family of β-barrel pore-forming exotoxins that are secreted by gram-positive bacteria. CDCs are secreted as water-soluble monomers of 50-70 kDa, that when bound to the target cell, form a circular homo-oligomeric complex containing as many as 40 (or more) monomers. Through multiple conformational chan… WebDec 1, 2001 · LLO, a pore-forming toxin of the thiol-activated cholesterol-dependent cytolysins (CDCs) family, is secreted as a soluble monomer that oligomerizes upon … how to lessen cortisol
CDD Conserved Protein Domain Family: Thiol_cytolysin
WebStreptolysin-O is a sulfhydryl-activated toxin which causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the Streptolysin-O protein undergoes a major conformation change, leading to its insertion in the host membrane and creation of an oligomeric pore complex. WebNov 18, 1996 · We present the first reported crystallization of a member of the thiol-activated family of protein toxins. Perfringolysin O, a virulence factor of Clostridium perfringens, has been crystallized in two different forms by the hanging drop vapor diffusion method. In one form the toxin crystallizes with PEG 20000 in the orthorhombic space … WebJan 15, 2000 · The thiol-activated cytolysin (TACY) family is a prominent group of bacterial toxins, of which streptolysin O (SLO) is the prototype. Members of this group are elaborated by more than 20 species of Gram-positive bacteria [1] ( Table 1 ) and are intimately involved in the pathogenesis of infections by species such as Arcanobacterium pyogenes ... josh hicks for congress